Targeted and reversible cancer cell-binding DNA nanoparticles

Targeted and reversible cancer cell-binding DNA nanoparticles

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DNA nanoparticles (DeNAno) produced by rolling circle replication of circular oligonucleotide templates are a novel format for the selection of cell-binding reagents from randomized libraries.DeNAno particles consist of several hundred concatemers and can leverage that multivalency to rawafricaonline.com bind to complex surfaces such as cells.In this study, an iterative bio-panning approach was used to recover particles that bound to the mouse pancreatic cancer line, Panc-02.These particles shared a primary sequence motif.

Hybridization of a locked nucleic acid complimentary to this motif both inhibited cell binding sophie allport bee curtains and released pre-bound DeNAno particles from the cells.The monomeric form of one of the selected sequences was unable to compete with the cognate particle, consistent with a low-affinity, but high-avidity, type interaction.DeNAno library selection against cancer or other cell types can, thus, yield novel cell binding agents without a priori knowledge of particular cell surface molecules.